Course Description:
This course is designed as a continuation of Biotechnology I with a greater emphasis on proteomics. Students will be introduced to the scientific concepts and advanced laboratory research techniques currently used in the field of biotechnology. Areas of study include proteomics, eukaryotic tissue culturing through hormone manipulation, synthesizing DNA and advanced PCR techniques.
Laboratories done in class:
Exploring Plant structure and function with Wisconsin Fast Plants
Plant Tissue Culture Techniques
Naturopathic Botanical Medicine: Making a Healing Salve
Bioprocessing with Bioreactors
Primer Design
Do You Have the PV92 Insert?
Mitochondrial DNA Control Region Sequence and Comparison
DNA Sequencing
Bioinformatics: Creating Phylogenetic Trees
Eukaryotic Culture Cell Counts
Caenorhabditis elegans: Observing, Culturing, and Caring for a Model Organism
Plasmid Mapping
Determining Unknown Protein Concentration Using the Bradford Protein Assay
Protein Fingerprinting
Western Blottting
Bacterial Titration
Determining the Concentration of Viruses
What is an Auxotroph?
Microbial Genomics: Complementation to Test Annotation of a Gene as Important for Proline Biosynthesis
District requested to modify course description