Course title

Biomedical Innovations II

Pre-requisite

Biomedical Innovations I

Course description

BioMedical Innovations II
Course Overview
This course is an extension of biomedical health technologies I and the career possibilities in the biotechnology field. Students will be introduced to past; present and future applications of Biotechnology and learn advanced lab procedures. Topics covered will include understanding laboratory procedures fundamental to biomedical research which include recombinant DNA; protein purification; cell and tissue culture. An emphasis will be placed on proteins as well as independent research. Additional topics include: communication skills; the history and development of the field of biomedical research; genetically modified organisms (GMOs); bioethics; and career possibilities in Biotechnology.

Emphasis is placed upon laboratory skills development and data analysis. We will follow the CTE standards for Biotechnology: Bio-Innovations. Students will achieve an understanding of the major principles of biotechnology through their use of problem solving skills and laboratory experiences. Students will become proficient at collecting data and problem solving skills.

Course Expectations
A foundation of knowledge from Biotechnology I is required so that students can build on their basic skills of biotechnology in the workplace and apply them to further studying advanced biotechnology techniques. Students will be expected to develop an independent research topic and conduct experiments in that area. .

Laboratory Activities
Laboratory skills are an essential component to the course and will constitute more than 25% of the course. It is imperative that purpose and proper procedure are known before entering the laboratory. There will be pre- and post-laboratory discussions and write-ups to help solidify the concepts. Students will properly follow laboratory procedures as they use new equipment and learn new techniques necessary to be successful in a laboratory setting. Students will maintain a scientific lab book and will be evaluated on proper laboratory techniques. Students will complete Biotechnology II with an independent research project of their choice. Students will present their research through a research poster.

Student Evaluation
A traditional grading scale (100-90 % = A; 89-80 % = B; 79-70 % = C; etc.) will be used for this course.

A test is given for each topic listed. The test will be modeled after the CTE standards and the Arizona state standards. Exams typically will have 35-50 multiple-choice questions and one essay question. Larger units may be divided into sections; with a test for each. The multiple choice questions are taken from the textbook as well as relevant questions from laboratory experiments. The essay question will be a performance based question that correlates with the concepts being tested. Reading quizzes and lab quizzes as well as skills tests will also be given throughout the course. Each semester grade is determined by the following:

Homework 15%
(e.g.; completed study guides; activity worksheets;
problem sets; writing abstracts; article reviews)
Laboratory Work 35%
(Scientific Lab Notebook)
Exams and quizzes 30%
(Final exams are worth 15% of your semester grade)
Final Research Project with Poster Presentation 20%

Textbook:
Daugherty; Ellyn.. Biotechnology;. Paradigm Publishing Inc. 2007.
Student Lab Book:
Daugherty; Ellyn.. Biotechnology;. Paradigm Publishing Inc. 2007.

Proposed Course Outline Subject to Change Labs/Activities Chapters
I. Past; Present & Future Applications of Biotechnology (Daugherty)
A. Biotechnology in the Future 1
Potential Products Historical application and future discoveries
Scientific fields relating to biotechnology
Exploring Careers Biotech Online Search: Finding ?Hot Jobs?
Bioethics: Cloning; Using Animals In Science Web Search: Monsanto & feeding the world
and Industry; Farmers & Rights Web Search: Examining a Code of Business Conduct
Activity: Design Logo for a company & write a company
Code of Business Conduct & Ethics

B. Scientific Method & Lab Safety 1
Lab Safety review Lab Safety Activities; Biohazard Simulations
Setting up a Scientific Notebook review Biotech Live- Biohazard (web search)
Maintaining an Inventory Inventory all glassware & maintain conditions throughout
the year.
Problem solving using the Scientific Method Lab-How Molecular Structure is Affected by Environmental
Change (Daugherty pg 29)
Lab- SOPs Micropipette & Balance Calibration & % Error
(Protein is Cash Lab Book)
Common Controls (positive & negative) Lab- DNA Gel Electrophoresis with Controls
(MSA adp P pg 457)
Or
Lab-Testing a Protocol: Extraction of Salicylic Acid from
Willow (Daugherty Ch 12)
Graphing (Advanced Excel Graphing) Graphing with Excel
(MSA Lab Book Ch 1)
Scientific Methodology in a Research Facility

C. Basic Separation & Amplification Methods
Biotechnology Equipment review Lab Using a Spectrophotometer to Check Copper Sulfate
Solutions (Daugherty Ch 3)
Introduction to Filtration
Introduction to Centrifugation Lab-Practicing with a Micro-Centrifuging;
(Daugherty Ch 3)
Introduction to Bioseparations
PCR Lab-Crime Scene Investigator PCR Basics Kit
(BioRad #166-2650EDU 205.00)

D. Laboratory Solutions 3
Concentrations Expressions & Calculations Lab-Measuring Small & Very Small Volumes in a Biotech Lab
(Daugherty pp 32; 35)
Basic Procedures Lab-Making Solutions of Differing Mass/Volume
(Daugherty pg 45)
Lab-Making solutions of Differing Molarity Concentrations
with the Spectrophotometer
(Daugherty pg 55)
Solutions to Support the Activity of
Biological Macromolecules Lab-Making Media for Bacteria Cell Culture (Media Prep)
(Daugherty pg 71)
Culture Media for Intact Cells Lab-Bacteria Cell Culture
(Daugherty pg 76)
Lab-Growing & Maintaining Healthy Cultures: Effects of
Chemicals & UV light on Bacterial Cultures
(MSA Lab Book Ch 1)

E. Quality Assays and Tests
The Components of an Assay Lab- Searching for Native Amylase Producing Bacteria
(Daugherty Ch 6)
Assay Development
Quality Tests & Assays
Technology Relating to Assays & Examples Lab-Testing Plant & Animal Samples for Hydrogen Peroxidase
(Daugherty pg 120 Ch 6)
Using Light
Using the Spectrophotometer Lab- Using the Spectrophotometer to Measure Protein
Constructing a Standard Curve Concentration
(MSA adp P pg 464)

F. Biotech Skill Standards Maintain Standards Mastery through Standards Skills Booklet

II. Introduction to Studying Proteins

A. Protein Structure 5
Protein Sequence & Mutations Lab ? Mutations
(MSA Ch 6)
Classes of Proteins: Structure & Function Lab-Chromatography-Separation of Proteins
(MSA Ch 6)
Proteins & Transport Across the Membrane Lab-Dialysis-Can a Protein Cross the Membrane?
(MSA Lab Book Ch 5)
Proteins used in Biotechnology

Bioethics Activity: Bioethics (Web Search)
Plasmid Technology Lab-Transformation DNALC Green Gene Colony
Transformation Kit Refill
(Carolina Refill 4-8 station # RC-211082C 21.00)

B. Enzymes 5; 7
Measurement with Metrics & Conversions Measurement Lab
(MSA Lab Book Ch 3)
Lab-The Action of Different Enzymes on Apple Juice
Production
(Daugherty Ch 5)

C. Protein PAGE & Purification
Native & SDS Page Lab- Characterizing Proteins by PAGE
Vertical Gels (Daugherty Ch 5) Lab-Separating and Identifying Proteins via SDS PAGE
(Daugherty Ch 5)
Separating Proteins using Ion Exchange Lab-GFP Chromatography Kit
Chromatography (BioRad: # 166-0005EDU 101.00)

C. Studying Antibodies 5
Structure and Function of Proteins Lab-Making a Protein
(MSA Lab Book Ch 6)
Antibodies Lab-Protein Separation and Identification Gel Electro
(MSA Lab Book Ch 6)
Biomanufacturing Proteins Thinking Like a Biotechnician

D. Advanced Protein Studies pGLO Kit SDS-PAGE Extension w/ vertical gel
(BioRad 166-0013EDU 85.00)

E. BIOINFORMATIC RESOURCES Identify & Utilize electronic databases/websites (NCBI)
To search for relationships between protein
Sequences http://www.ncbi.nlm.nih.gov/

III. Biotechnology in Medicine

A. Identifying a Potential Biotechnology Product 6. 8; 9
Sources of Potential Products Biotech Online-Amazon Hide and Seek
Bringing a Biotechnology Product to Market Activity: R & D for new Product: Calculate Cost;
Target Market; Time
Product Quality Control
Looking for New Products in Nature Lab-Isolating Horseradish Peroxidase from
Radish Root
(Daugherty Ch 6)

B. Drug Discovery 12
Using a Spectrophotometer to Detect Molecules Lab-Better Use of the Spectrophotometer
(MSA Lab Book Ch 12)
Using the Spectrophotometer to Measure Protein Lab-Determining the Purity & Concentration of DNA
Concentration Samples
(Daugherty Ch 11)
Using the Spectrophotometer in Medicine

C. Recombinant Biotechnology 8
Creating Pharmaceuticals by ProteiN/Antibody Lab-Immunodetection Procedures to Detect Myosin
Engineering Light Chain
(BioRad #166-2800EDU 187.00 W. Blot)

An Overview of Genetic Engineering
Plasmid Technology
Polymerase Chain Reaction PCR Lab-PCR simulation
(MSA Lab Book Ch 8)

D. Plant Biotechnology 10; 11
Basic Plant Propagation Lab-Asexual Plant Propagation through Leaf & Stem
Cuttings
(Daugherty Ch 11)
Lab-Wisconsin Fast Plants: How fast is fast?
(Daugherty Ch 10)
Basic Plant Structure; Growth & Development Lab: Flower & Seed Morphology /Dissection
(Daugherty Ch 10)
Cloning by Plant Tissue Culture Lab-GMO Investigator Kit
GMO are they a good thing? (BioRad # 166-2550EDU 219.00)
Plant Tissue Culture Activity: Patents on GMOs (web search)
Applications of Biotechnology in Agriculture & Horticulture
Isolating DNA from Plant Cells Lab-DNA extraction from Plant Cells with Gel Electro
(MSA Lab book Ch 10)
E. Bioethics
Cloning
Stem Cells http://learn.genetics.utah.edu.
Eugenics Web based exercise on Cloning
Gene Therapy Web-Eugenic Movement
ELSI Web-DOLAN
Human Genome Project

E. BIOINFORMATIC RESOURCES Identify & Utilize electronic databases/websites (NCBI)
To search for relationships between protein
Sequences http://www.ncbi.nlm.nih.gov/

IV Career Pathways in Biomedical Health Technologies

A. Survey the Biotechnology Field Web-Search Activity & Presentation
Job Search Skills Web-Search Laws and Restrictions
Employability Skills Write a Resume
B. Develop Individual Career Plan Fill out Sample Applications
Prepare for Employment Write Cover Letter
Oral & Written Communication Skills Write a Letter of Recommendation
Mock Interviews
C. Quality Assurance
How to handle complaints; harassment; bioethics
D. Work-Based Learning

V. Advanced Biotechnology Independent Research
To be started 2nd semester; possible topics 14
DNA Sequencing
Genomics
Nanotechnology
Cloning
Plant/Animal Cell Cultures
Final Project-Research Poster & Presentation

Dress for Success

TYPICAL LAB from ?Biotechnology- Science for the New Millennium? by Ellyn Daugherty

Laboratory 3g Making Solutions of Differing Molarity Concentrations
The concentration of many solutions is reported as moles/liter (mol/L or M the M is spoken ?molar)or some fraction of those units. This concentration measurement is called mo1arIy. Molarity is sometimes a challenging concept to understand. However; with your recently acquired solution preparation skills; you will see that making molar solutions requires only one extra calculation.
To understand how co make a solution of a given molarity; you must know what a ?mole? is.
A mole of a compound is equal to 6.02x1023 molecules; but that is not really a very useful number. So; in biotech; it is easier to use this definition: The unit ?1 mole? is the mass; In grains; equal to the molecular weight (MW); also called ?formula weights (FW); of the substance (seeFigure 3.16). The FW can be determined by using a Periodic Table or by adding the atomic weights of the atoms in the molecule. An easy way; though; is to just read the label of a chemical reagent bottle; which Lists the ?MW or FW? The molecular weight of NaC1 is 58.5 atomic mass units (amu) since the Na atom weighs 23 amu; and a Cl atom weighs 35.5 amu. Molarity concentrations are reported
as the number of moles per liter (mol/L or AO. If the concentration Is very low;
then the concentration could be reported in millrnolesllirer (mmol. or mM). If you wanted a l-M NaC1 solution; you would measure out I mole of NaCI (585
g) and dissolve it in Water to a total volume of I L This gives you 1 mole of NaCI per liter of solution; I M NaCI. A liter of solution Is a large volume
for most research and development purposes. In research and development
labs; mL or L quantities are usually used. To determine how to mix up a
smaller volume of a solution of some molarity.
Follow the example below.
Multiply the volume desired (L) by the concentration (molarity) desired (mol/L); as you did in the mass volume calculations. Then; multiply the result by the compound?s molecular weight (g/mol) to account for measuring in moles; as in the following equation: Molarity Concentration Equation
volume x molarity x molecular weight - grams of solute to be dissolved in
wanted desired of the solute solvent to the final desired volume
(L) (molli) (g/mol) Convert smaller or larger units to these as necessary. The ?L? units cancel out and the ?mol? units cancel out; leaving the mass in grams of the solute needed to make the solution.
Remember that the math is easiest if the units of measure can be cancelled during multiplication. Use the B S Rule to convert between these metric units of measure:
L mL uL
Km m cm mm in
Kgg mgg
M m M uM

Purpose
To make copper sulfate pentahydrate solutions of different volumes and molar concentrations
Materials
Caution: Wear goggles and gloves when measuring chemicals.
Balance; analytical Deionized water
Balance; tabletop milligram Permanent lab marker pens
Weigh paper; 7.6x7.6 cm Tubes; glass; 13x100 mm
Weigh boat; 3.5?x3.5? Peg racks for 10?13 mm tubes
Lab scoops Pipets; 5 mL
Cupric sulfate 5-hydrate Pipete pump; green
Tubes; 15 mL; capped Spectrophotometer; Spectronic 20 D+
Tube racks for 15 mL tubes

Procedure
* Label all cubes with the concentrate ton 0f the sample; your Initials;
and the date
? Review the use of the balance and weigh boats before beginning.
1. For tubes numbered 1 through5; prepare the solutions listed in Table 3.18. Use the Molarity Concentration equation (refer to the discussion at the beginning of this lab activity)
to determine the mass of CuSO4 to measure in order to give the right concentration and volume in each sample. Make a reaction matrix to record the volume; mass; and mathematical calculations for each solution (see Table 319). Show all units of measure and draw a picture to describe how to make each solution Be sure to make the calculations and the
Diagram columns large enough for their contents

Table 3.18. Molar Solutions To be Made
Tube No Solution To Be Prepared
I 5 mL of 1.0 M CuSO4
2 5 mL of 0.5 M CuSO4
3 5 mL of 0.1 M CuSO4
4 5 mL of 0.05 M CuSO4
5 5 mL of 0.01 M CuSO4

Table 3.19 Concentrations of the CuSO4 Solution Tubes
Tube # Total Volume (mL) Mass of CuSo4(g) Calculations Diagram of Solution Prep
1 . . . .
2
3
4
5

2. Prepare the solutions using deionized water in Labeled 15-mL capped; conical tubes Add the solute flint; then add water to the total desired volume. Mix until the solute is dissolved

Data Analysis and Conclusion
Are the differences in concentration of your five tubes obvious In one tube verses another?

Describe any differences and explain why the differences are observed °f any volumes or colors are obviously wrong; dispose and remake them; Compare the colors and volumes of your samples too(he In the class. Describe the impact if the final volume is Incorrect; What will happen
to the concentration of copper sulfate in the samples?

Thinking Like a Biotechnician

1. Each of the tubes; °n this activity was made from scratch by measuring out a specific mass of dry chemicals and mixing it with a specified volume of solvent.
Suggest a method to make a O.5-M solution from the l-M solution
Also; suggest a way to make a O.l M solution from the 0.5 - M solution.
2. Calculate the mass/ volume concentration °n each tube and the % mass/volume concentration in each tube and record these data in a table similar to Table 3.2O. Be sure lo make the
'calculations' column wide enough for the equations.

Table 3.20 Concentration Equivalents

Molar Concentrations of Each Tube Concentrations(g/mL) Concentration(%) Calculations
5 mL of 1.0 M CuSO4 .
5 mL of 0.5 mL CuSO4
5 mL of 0.1 mL CuSO4
5 mL of 0.05 mL CuSO4
5 mL of 0.01 mL CuSO4

3. Following the protocol presented In Lab 3e; use the spectrophotometer to
check the copper sulfate solution's concentration and preparation.
4. Complete the Making Solution Review Sheet No. 3 that follows

CAROLINA
Section Lab Need to Order Cost
IIA Refill DNALC Green Gene Colony Transformation Kit # RC-211082C 21.00

BIORAD
Section Lab Need to Order Cost
IC Crime Scene Investigator PCR Basics Kit
(BioRad #166-2650EDU ) 205.00
IIC GFP Chromatography Kit
(BioRad: # 166-0005EDU) 101.00
IID pGLO Kit SDS-PAGE Extension w/ vertical gel
(BioRad 166-0013EDU ) 85.00
IIIC Immunodetection Procedures to Detect Myosin Light Chain
(BioRad #166-2800EDU W. Blot) 187.00
IIID GMO Investigator Kit (BioRad # 166-2550EDU ) 219.00

PROTEIN IS CASH
Section Lab Need to Order Cost
IB Lab- SOPs Micropipette & Balance Calibration & % Error
(Protein is Cash Lab Book)
NONE 00.00

DAUGHERTY
Section Lab Need to Order Cost
IB Lab-How Molecular Structure is Affected by Environmental Change (Daugherty pg 29)

IC Lab-Testing a Protocol: Extraction of Salicylic Acid from Willow (Daugherty Ch 12)

IC Lab Using a Spectrophotometer to Check Copper Sulfate Solutions (Daugherty Ch 3)

IC Lab-Practicing with a Micro-Centrifuging;
(Daugherty Ch 3)

ID Lab-Measuring Small/Very Small Volumes in a Biotech Lab
(Daugherty pp 32; 35)

ID Lab-Making Solutions of Differing Mass/Volume
(Daugherty pg 45)

ID Lab-Making solutions of Differing Molarity Concentrations with the Spectrophotometer
(Daugherty pg 55)

ID Lab-Making Media for Bacteria Cell Culture (Media Prep)
(Daugherty pg 71)

ID Lab-Bacteria Cell Culture
(Daugherty pg 76)

IE Lab- Searching for Native Amylase Producing Bacteria
(Daugherty Ch 6)

IE Lab-Testing Plant & Animal Samples for Hydrogen Peroxidase
(Daugherty pg 120 Ch 6)

IIB Lab-The Action of Different Enzymes on Apple Juice Production
(Daugherty Ch 5)

IIC Lab- Characterizing Proteins by PAGE
(Daugherty Ch 5)

IIC Lab-Separating and Identifying Proteins via SDS PAGE
(Daugherty Ch 5)

IIIA Lab-Isolating Horseradish Peroxidase from Radish Root
(Daugherty Ch 6)

IIIB Lab-Determining the Purity & Concentration of DNA Samples
(Daugherty Ch 11)

IIID Lab-Asexual Plant Propagation through Leaf & Stem Cuttings
(Daugherty Ch 11)

IIID Lab-Wisconsin Fast Plants: How fast is fast?
(Daugherty Ch 10)

IIID Lab: Flower & Seed Morphology /Dissection
(Daugherty Ch 10)

MSA LAB BOOK

IB Lab- DNA Gel Electrophoresis with Controls
(MSA adp P pg 457)

IB Graphing with Excel
(MSA Lab Book Ch 1)

ID Lab-Growing & Maintaining Healthy Cultures: Effects of
Chemicals & UV light on Bacterial Cultures
(MSA Lab Book Ch 1)

IE Lab- Using the Spectrophotometer to Measure Protein Concentration
(MSA adp P pg 464)

IIA Lab ? Mutations
(MSA Ch 6)

IIB Measurement Lab
(MSA Lab Book Ch 3)

IIC Lab-Dialysis-Can a Protein Cross the Membrane?
(MSA Lab Book Ch 5)

IIC Lab-Chromatography-Separation of Proteins
(MSA Ch 6)

IIIB Lab-Better Use of the Spectrophotometer
(MSA Lab Book Ch 12)

IIIC Lab-PCR simulation
(MSA Lab Book Ch 8)

IIID Lab-DNA extraction from Plant Cells with Gel Electro
(MSA Lab book Ch 10)

Equipment

New
Vertical Gel tanks 390.00

Wish List:
BioRad Spectrophotometer 4376.00
Mini Incubation Oven BioRad 367.00

School country

United States

School state

Arizona

School city

Scottsdale

High school

Saguaro High School

School / district Address

8500 E. Jackrabbit Road

School zip code

85250

Date submitted

10/27/2010

Approved

Yes

Approved competency code

• CTE
• Career and technical education

• LADV
• Advanced science

• LBIO
• Biology

Approved date

11/1/2010

Online / Virtual

No