Course title

Bioscience 3

Pre-requisite

Bioscience 2, Biology

Course description

BioScience 3 is a biological research methods course. Students will be expected to design their own independent research project; collect and analyze data; and present it to their peers. These projects will be entered in Andrada’s own science fair; Vail Pride Day; and the Southern Arizona Regional Science and Engineering Fair (SARSEF); and the Arizona-Nevada Academy of Science conference in April.
Whole-class projects include DNA barcoding of Arizona reptiles in cooperation with the Arizona-Sonora Desert Museum; Alu retrotransposon detection and subsequent statistical analysis of allele frequencies; and ecological survey and species quantification of on-campus study sites. In addition; student will be required to culture and maintain bacterial and eukaryotic cell cultures for classroom or individual use.

This course is offered for dual credit through the University of Arizona’s Molecular and Cellular Biology department.

Bioscience 3 Labs

Labs include:

· Identifying macromolecules (carbohydrates; lipids; proteins)

· Affects of pH on enzyme (catalase) function

· Affects of temperature on enzyme (catalase) function

· Using enzymes (amylase; cellulase) to alter fabric color

· Using a spectrophotometer to determine solution concentration (Beer’s Law)

· Comparing Biuret; Lowery; and Bradford assays

· Using fungal enzymes (cellobiase) to improve biofuel production

· Student-designed investigation—which fungi have the most cellobiase

· DNA barcoding of insects (including PCR and agarose electrophoresis review and)

· Bioinformatics search (BLAST) of insect barcode sequences

· Cell biology review (structure and function)

· Measuring human epithelial cell diameters

· Using cell diameter to plot normal distribution

· Plant anatomy/physiology overview

· Student-designed investigation—factors affect Wisconsin Fast Plant growth (poster presentation)

· Demonstrating Koch’s Postulates of disease transmission using yogurt and milk

· Alu Retrotransposon Lab using standard primers for PV92 locus

· Design primers loci other than PV92

· Chi-squared analysis of Alu data

· DNA quantification using UV-Vis spectrophotometer

· Chicken wing dissection

· SDS-PAGE electrophoresis of chicken proteins

· Starting; maintaining; passaging and freezing bacteria-free yeast culture

· Using a hemocytometer to determine cell density

· Using a spectrophotometer to determine cell density

BioScience 3 is a Career and Technical Education (CTE) course and curriculum is based on the Arizona State CTE standards:

Standard 1.0 Maintain a safe work environment
Standard 2.0 Demonstrate understanding and relevance of standard laboratory operating procedures

Standard 3.0 Demonstrate critical thinking and scientific problem-solving skills in scientific inquiry

Standard 4.0 Demonstrate research and investigative skills

Standard 5.0 Demonstrate ethical and legal conduct in job-related activities

Standard 6.0 Adhere to quality assurance procedures

Standard 7.0 Adhere to quality control procedures

Standard 8.0 Understand the role of living organisms in bioscience research

Standard 9.0 Demonstrate basic lab skills in the use of equipment and instrumentation

Standard 10.0 Demonstrate understanding and knowledge of cell biology techniques

Standard 11.0 Demonstrate understanding and knowledge of microbiology skills

Standard 12.0 Demonstrate understanding and knowledge of protein techniques

Standard 13.0 Demonstrate knowledge of material preparation and storage

Standard 14.0 Demonstrate use of bioinformatics resources

Standard 15.0 Demonstrate understanding and knowledge of nucleic acid techniques

Standard 16.0 Demonstrate understanding and knowledge of scientific measurements

Standard 17.0 Engage in occupational-specific work-based learning experience in chosen career field (e.g.; biomedical; bioenvironmental; bio-innovations

School Country

United States

School state

Arizona

School city

Tucson

High school

Andrada Polytechnic

School Address

12960 S. Houghton Rd

School zip code

85747

Requested competency code

Lab Science

Date submitted

05/27/2015

Approved

Yes

Approved competency code

• LBIO
• Biology

Approved date

06/15/2015

Online / Virtual

No