Course title

Pre-requisite

Course description

The Capstone: Biochemistry course is intended to introduce the student to common techniques in biochemical and biomedical research. Topics and lab techniques to be covered may include; but not be limited to an introduction to organic chemistry; spectrophotometric methods; enzyme and protein analysis; molecular biology (including DNA extraction and analysis; subcloning techniques and PCR); microbiology; and protein purification. The student will also be expected to complete a number of projects including presentations of research topics.

Introduction to Organic Chemistry
Carbon atom
Structure and Nomenclature of Hydrocarbons
Alkanes
Alkenes
Alkynes
Aromatics
Isomerism and Sterochemistry of Organic Molecules
Reactions of Hydrocarbons
Basic Organic Techniques
Crystallization & Extraction
Synthesis of Aspirin
Preparation of a Soap
Spectrophotometric Methods
UV/Vis Spectrophotometer
Beer's Law
Creation of Standard Curves
Graphing using EXCEL
Kinetics using Spectrophotometers
Intro to other Spectrophotometric Methods (IR;Mass Spec; etc.)
Microbiology
Sterile Technique
Preparation of Materials
Use of Autoclave
Agar Plates and Media
Collection of Samples
Streaking of Plates
Isolation of Bacterial Colonies
Gram Positive/Gram Negative Identification
Preparation of Stock Bacteria
Molecular Biology
DNA Techniques
Micropipetting Techniques
Dilution of Samples
Agarose Gel Electrophoresis
Preparation of Agarose Gels and Solutions
Plasmids
Purification of Plasmids from Bacteria
Restriction Enzyme Digests
Subcloning of DNA fragments
Transformation of GFP Plasmid into E. coli
PCR Reaction Procedures
Analysis of PCR Products
Extraction of Genomic DNA Procedures
Introduction to Bioinformatics
Protein and Enzyme Analysis
Protein Concentrations
Biuret; Bradford and Direct Absorbance
Enzyme Activity
Endpoint and Kinetic
Calculation of Enzyme Activity
Protein Purification Techniques
Introduction to Protein Purification (Extraction of Casein from Milk)
Buffer Preparation
Cell Extract/Lysis of Cells (bacterial and yeast)
Clarification of Extract
Centrifugation
Filtration
Protein Precipitation Techniques
Ammonium Sulfate; Heat and Acid Precipitation
Liquid Chromatography Techniques
Ion Exchange; HIC; Affinity and Gel Filtration
Small scale GFP Purification
Large Scale GFP Purification (from large scale growth of transformed E. coli cells)

School country

School state

School city

High school

School / district Address

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Requested competency code

Date submitted

Approved

Approved competency code

• LINT
• Integrated science

Approved date

Online / Virtual