Course title

Introduction to Biotechnology

Pre-requisite

Biology

Course description

 https://tp.sduhsd.net/documents/Counseling/Course%20Profiles/Science/Introduction%20To%20Biotechnology.pdf 

https://tp.sduhsd.net/Counseling/Course-Profiles/index.html

This is what we hope to do with the Intro to Biotechnology classes this year: • learn how to properly use a variety of micropipettes; vortexer; centrifuge; gel electrophoresis apparatus; pcr machine; transilluminator; incubator; vertical protein gel apparatus; column chromotography; and more. • Collect your own DNA to take home in a tube • Collect cheek cells or hair root cells and make copies of a section of your own DNA (this is called PCR; polymerase chain reaction) • Run a gel (gel electrophoresis) to see the VNTR DNA fingerprint of your own DNA • Do PCR and gel electrophoresis with foods you bring in from home to determine if they are genetically modified organisms (GMO) or not • Work with bacteria; yeast; fruit flies (Drosophila melanogaster); nematodes (C. elegans); plants and seeds of various species • Induce bacteria to take up a gene that will allow them to be fluorescent and resistant to an antibiotic • Purify the green fluorescent protein from live bacteria and conduct protein chromatography • Plant and compare a variety of species with GMO and non GMO seeds • Conduct a simulated AIDS detection. This is a technique called enzyme-linked immunosorbent assay (ELISA); or enzyme immunoassay (EIA) • 

School Country

United States

School state

California

School city

Encinitas

School Address

3710 Del Mar Heights Road, San Diego, CA

School zip code

92130

Requested competency code

Lab Science

Date submitted

11/9/2018

Approved

Yes

Approved competency code

• LBIO
• Biology

Approved date

11/9/2018

Online / Virtual

No